Mapping Epitopes of the Plasmodium vivax Duffy Binding Protein with Naturally Acquired Inhibitory Antibodies
نویسندگان
چکیده
منابع مشابه
Conserved and variant epitopes of Plasmodium vivax Duffy binding protein as targets of inhibitory monoclonal antibodies.
The Duffy binding protein (DBP) is a vital ligand for Plasmodium vivax blood-stage merozoite invasion, making the molecule an attractive vaccine candidate against vivax malaria. Similar to other blood-stage vaccine candidates, DBP allelic variation eliciting a strain-specific immunity may be a major challenge for development of a broadly effective vaccine against vivax malaria. To understand wh...
متن کاملNaturally acquired Duffy-binding protein-specific binding inhibitory antibodies confer protection from blood-stage Plasmodium vivax infection.
Individuals residing in malaria-endemic regions acquire protective immunity after repeated infection with malaria parasites; however, mechanisms of protective immunity and their immune correlates are poorly understood. Blood-stage infection with Plasmodium vivax depends completely on interaction of P. vivax Duffy-binding protein (PvDBP) with the Duffy antigen on host erythrocytes. Here, we perf...
متن کاملConserved and variant epitopes of Plasmodium vivax Duffy binding protein as 1 targets of inhibitory monoclonal antibodies
3 Running title: Inhibitory anti-DBP monoclonal antibodies 4 5 Francis B. Ntumngia, Jesse Schloegel, Samantha J. Barnes, Amy M. McHenry, Sanjay Singh 6 Christopher L. King , and John H. Adams 7 8 9 University of South Florida, Tampa, FL, United States 10 Case Western Reserve University, Cleveland, OH, United States 11 Veterans Affairs Medical Center, Cleveland, OH, United States 12 Gennova Biop...
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A vaccine to eliminate malaria would need a multi-stage and multi-species composition to achieve robust protection, but the lack of knowledge about antigen targets and mechanisms of protection precludes the development of fully efficacious malaria vaccines, especially for Plasmodium vivax (Pv). Pregnant women constitute a risk population who would greatly benefit from a vaccine preventing the a...
متن کاملOptimized Method for Purification of Expressed Plasmodium Vivax Duffy Binding Protein-II (PvDBP-II): Implication for Vivax Malaria Vaccine Development
Background: The purity and correct folding of a recombinant protein is critical for any structural, biochemical and vaccine design studies. Plasmodium vivax Duffy binding protein-II is a leading vaccine candidate for vivax malaria. In the present study, the purification process of recombinant DBP-IX (a variant form of PvDBP-II) was optimized to achieve the highest yield and purity. Moreover, ...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2010
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.01036-09